A novel and highly efficient method for genetic transformation of fungi employing shock waves.
Identifieur interne : 000398 ( Main/Exploration ); précédent : 000397; suivant : 000399A novel and highly efficient method for genetic transformation of fungi employing shock waves.
Auteurs : Denis Maga A-Ortíz [Mexique] ; Nancy Coconi-Linares ; Elizabeth Ortiz-Vazquez ; Francisco Fernández ; Achim M. Loske ; Miguel A. G Mez-LimSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2013.
Descripteurs français
- KwdFr :
- Biologie moléculaire (instrumentation), Biologie moléculaire (méthodes), Champignons (génétique), Contrainte mécanique (MeSH), Génétique microbienne (instrumentation), Génétique microbienne (méthodes), Spores fongiques (génétique), Techniques de transfert de gènes (instrumentation), Transformation génétique (MeSH).
- MESH :
- génétique : Champignons, Spores fongiques.
- méthodes : Biologie moléculaire, Génétique microbienne.
- instrumentation : Biologie moléculaire, Contrainte mécanique, Génétique microbienne, Techniques de transfert de gènes, Transformation génétique.
English descriptors
- KwdEn :
- MESH :
- genetics : Fungi, Spores, Fungal.
- instrumentation : Gene Transfer Techniques, Genetics, Microbial, Molecular Biology.
- methods : Genetics, Microbial, Molecular Biology.
- Stress, Mechanical, Transformation, Genetic.
Abstract
Genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. However, available protocols to transform fungi are inefficient, laborious and have low reproducibility. We report the use of underwater shock waves as a novel method to transform filamentous fungi. An experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous DNA. The device was successfully tested in Aspergillus niger, Fusarium oxysporum, Trichoderma reesei and Phanerochaete chrysosporium. The transformation frequency per number of conidia was between two and four orders of magnitude higher in comparison to previously published methods. For example, the frequency of transformation in A. niger was improved up to 5400-fold as compared with Agrobacterium protocols. Transformation was verified by expression of the green fluorescent protein, PCR and Southern blot. Our method offers new possibilities for fast, easy and efficient genetic manipulation of diverse fungal species.
DOI: 10.1016/j.fgb.2013.03.008
PubMed: 23583899
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A novel and highly efficient method for genetic transformation of fungi employing shock waves.</title>
<author><name sortKey="Maga A Ortiz, Denis" sort="Maga A Ortiz, Denis" uniqKey="Maga A Ortiz D" first="Denis" last="Maga A-Ortíz">Denis Maga A-Ortíz</name>
<affiliation wicri:level="1"><nlm:affiliation>Departamento de Ingeniería Genética de Plantas, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato, Mexico.</nlm:affiliation>
<country xml:lang="fr">Mexique</country>
<wicri:regionArea>Departamento de Ingeniería Genética de Plantas, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato</wicri:regionArea>
<wicri:noRegion>Guanajuato</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Coconi Linares, Nancy" sort="Coconi Linares, Nancy" uniqKey="Coconi Linares N" first="Nancy" last="Coconi-Linares">Nancy Coconi-Linares</name>
</author>
<author><name sortKey="Ortiz Vazquez, Elizabeth" sort="Ortiz Vazquez, Elizabeth" uniqKey="Ortiz Vazquez E" first="Elizabeth" last="Ortiz-Vazquez">Elizabeth Ortiz-Vazquez</name>
</author>
<author><name sortKey="Fernandez, Francisco" sort="Fernandez, Francisco" uniqKey="Fernandez F" first="Francisco" last="Fernández">Francisco Fernández</name>
</author>
<author><name sortKey="Loske, Achim M" sort="Loske, Achim M" uniqKey="Loske A" first="Achim M" last="Loske">Achim M. Loske</name>
</author>
<author><name sortKey="G Mez Lim, Miguel A" sort="G Mez Lim, Miguel A" uniqKey="G Mez Lim M" first="Miguel A" last="G Mez-Lim">Miguel A. G Mez-Lim</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23583899</idno>
<idno type="pmid">23583899</idno>
<idno type="doi">10.1016/j.fgb.2013.03.008</idno>
<idno type="wicri:Area/Main/Corpus">000371</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000371</idno>
<idno type="wicri:Area/Main/Curation">000371</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000371</idno>
<idno type="wicri:Area/Main/Exploration">000371</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A novel and highly efficient method for genetic transformation of fungi employing shock waves.</title>
<author><name sortKey="Maga A Ortiz, Denis" sort="Maga A Ortiz, Denis" uniqKey="Maga A Ortiz D" first="Denis" last="Maga A-Ortíz">Denis Maga A-Ortíz</name>
<affiliation wicri:level="1"><nlm:affiliation>Departamento de Ingeniería Genética de Plantas, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato, Mexico.</nlm:affiliation>
<country xml:lang="fr">Mexique</country>
<wicri:regionArea>Departamento de Ingeniería Genética de Plantas, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato</wicri:regionArea>
<wicri:noRegion>Guanajuato</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Coconi Linares, Nancy" sort="Coconi Linares, Nancy" uniqKey="Coconi Linares N" first="Nancy" last="Coconi-Linares">Nancy Coconi-Linares</name>
</author>
<author><name sortKey="Ortiz Vazquez, Elizabeth" sort="Ortiz Vazquez, Elizabeth" uniqKey="Ortiz Vazquez E" first="Elizabeth" last="Ortiz-Vazquez">Elizabeth Ortiz-Vazquez</name>
</author>
<author><name sortKey="Fernandez, Francisco" sort="Fernandez, Francisco" uniqKey="Fernandez F" first="Francisco" last="Fernández">Francisco Fernández</name>
</author>
<author><name sortKey="Loske, Achim M" sort="Loske, Achim M" uniqKey="Loske A" first="Achim M" last="Loske">Achim M. Loske</name>
</author>
<author><name sortKey="G Mez Lim, Miguel A" sort="G Mez Lim, Miguel A" uniqKey="G Mez Lim M" first="Miguel A" last="G Mez-Lim">Miguel A. G Mez-Lim</name>
</author>
</analytic>
<series><title level="j">Fungal genetics and biology : FG & B</title>
<idno type="eISSN">1096-0937</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungi (genetics)</term>
<term>Gene Transfer Techniques (instrumentation)</term>
<term>Genetics, Microbial (instrumentation)</term>
<term>Genetics, Microbial (methods)</term>
<term>Molecular Biology (instrumentation)</term>
<term>Molecular Biology (methods)</term>
<term>Spores, Fungal (genetics)</term>
<term>Stress, Mechanical (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Biologie moléculaire (instrumentation)</term>
<term>Biologie moléculaire (méthodes)</term>
<term>Champignons (génétique)</term>
<term>Contrainte mécanique (MeSH)</term>
<term>Génétique microbienne (instrumentation)</term>
<term>Génétique microbienne (méthodes)</term>
<term>Spores fongiques (génétique)</term>
<term>Techniques de transfert de gènes (instrumentation)</term>
<term>Transformation génétique (MeSH)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term>
<term>Spores, Fungal</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Champignons</term>
<term>Spores fongiques</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Gene Transfer Techniques</term>
<term>Genetics, Microbial</term>
<term>Molecular Biology</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetics, Microbial</term>
<term>Molecular Biology</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biologie moléculaire</term>
<term>Génétique microbienne</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Stress, Mechanical</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Biologie moléculaire</term>
<term>Contrainte mécanique</term>
<term>Génétique microbienne</term>
<term>Techniques de transfert de gènes</term>
<term>Transformation génétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. However, available protocols to transform fungi are inefficient, laborious and have low reproducibility. We report the use of underwater shock waves as a novel method to transform filamentous fungi. An experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous DNA. The device was successfully tested in Aspergillus niger, Fusarium oxysporum, Trichoderma reesei and Phanerochaete chrysosporium. The transformation frequency per number of conidia was between two and four orders of magnitude higher in comparison to previously published methods. For example, the frequency of transformation in A. niger was improved up to 5400-fold as compared with Agrobacterium protocols. Transformation was verified by expression of the green fluorescent protein, PCR and Southern blot. Our method offers new possibilities for fast, easy and efficient genetic manipulation of diverse fungal species. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23583899</PMID>
<DateCompleted><Year>2013</Year>
<Month>10</Month>
<Day>24</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>56</Volume>
<PubDate><Year>2013</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Fungal genetics and biology : FG & B</Title>
<ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>A novel and highly efficient method for genetic transformation of fungi employing shock waves.</ArticleTitle>
<Pagination><MedlinePgn>9-16</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fgb.2013.03.008</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S1087-1845(13)00063-7</ELocationID>
<Abstract><AbstractText>Genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. However, available protocols to transform fungi are inefficient, laborious and have low reproducibility. We report the use of underwater shock waves as a novel method to transform filamentous fungi. An experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous DNA. The device was successfully tested in Aspergillus niger, Fusarium oxysporum, Trichoderma reesei and Phanerochaete chrysosporium. The transformation frequency per number of conidia was between two and four orders of magnitude higher in comparison to previously published methods. For example, the frequency of transformation in A. niger was improved up to 5400-fold as compared with Agrobacterium protocols. Transformation was verified by expression of the green fluorescent protein, PCR and Southern blot. Our method offers new possibilities for fast, easy and efficient genetic manipulation of diverse fungal species. </AbstractText>
<CopyrightInformation>Copyright © 2013 Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Magaña-Ortíz</LastName>
<ForeName>Denis</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Departamento de Ingeniería Genética de Plantas, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato, Mexico.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Coconi-Linares</LastName>
<ForeName>Nancy</ForeName>
<Initials>N</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ortiz-Vazquez</LastName>
<ForeName>Elizabeth</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Fernández</LastName>
<ForeName>Francisco</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y"><LastName>Loske</LastName>
<ForeName>Achim M</ForeName>
<Initials>AM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gómez-Lim</LastName>
<ForeName>Miguel A</ForeName>
<Initials>MA</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>04</Month>
<Day>10</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Fungal Genet Biol</MedlineTA>
<NlmUniqueID>9607601</NlmUniqueID>
<ISSNLinking>1087-1845</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018014" MajorTopicYN="N">Gene Transfer Techniques</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005827" MajorTopicYN="N">Genetics, Microbial</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008967" MajorTopicYN="N">Molecular Biology</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013314" MajorTopicYN="Y">Stress, Mechanical</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year>
<Month>02</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2013</Year>
<Month>03</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2013</Year>
<Month>03</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>4</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2013</Year>
<Month>4</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2013</Year>
<Month>10</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23583899</ArticleId>
<ArticleId IdType="pii">S1087-1845(13)00063-7</ArticleId>
<ArticleId IdType="doi">10.1016/j.fgb.2013.03.008</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Mexique</li>
</country>
</list>
<tree><noCountry><name sortKey="Coconi Linares, Nancy" sort="Coconi Linares, Nancy" uniqKey="Coconi Linares N" first="Nancy" last="Coconi-Linares">Nancy Coconi-Linares</name>
<name sortKey="Fernandez, Francisco" sort="Fernandez, Francisco" uniqKey="Fernandez F" first="Francisco" last="Fernández">Francisco Fernández</name>
<name sortKey="G Mez Lim, Miguel A" sort="G Mez Lim, Miguel A" uniqKey="G Mez Lim M" first="Miguel A" last="G Mez-Lim">Miguel A. G Mez-Lim</name>
<name sortKey="Loske, Achim M" sort="Loske, Achim M" uniqKey="Loske A" first="Achim M" last="Loske">Achim M. Loske</name>
<name sortKey="Ortiz Vazquez, Elizabeth" sort="Ortiz Vazquez, Elizabeth" uniqKey="Ortiz Vazquez E" first="Elizabeth" last="Ortiz-Vazquez">Elizabeth Ortiz-Vazquez</name>
</noCountry>
<country name="Mexique"><noRegion><name sortKey="Maga A Ortiz, Denis" sort="Maga A Ortiz, Denis" uniqKey="Maga A Ortiz D" first="Denis" last="Maga A-Ortíz">Denis Maga A-Ortíz</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000398 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000398 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PhanerochaeteV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:23583899 |texte= A novel and highly efficient method for genetic transformation of fungi employing shock waves. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23583899" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a PhanerochaeteV1
This area was generated with Dilib version V0.6.37. |